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VOL. 6, ISSUE 4 (2021)
In vitro propagation and phytochemical screening in coleus amboinicus
Authors
Manju M George
Abstract
Coleus amboinicus (Lour.) (syn. Plectranthus amboinicus), commonly known as Indian borage or Mexican mint, is a therapeutically important aromatic medicinal plant belonging to the family Lamiaceae. This manuscript comprehensively reviews the in vitro propagation techniques and phytochemical profiles of C. amboinicus, emphasizing its potential for sustainable production and pharmaceutical applications. Micropropagation protocols utilizing Murashige and Skoog (MS) medium supplemented with various concentrations of plant growth regulators including 6-benzylaminopurine (BAP), naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) have been successfully established for explant sterilization, callus induction, shoot proliferation, and root development. Optimal shoot multiplication has been achieved using MS medium with 0.4 mg/L BAP, while rooting is best accomplished on half-strength MS medium. Phytochemical screening reveals the presence of diverse bioactive compounds including alkaloids, flavonoids, tannins, saponins, phenolic compounds, terpenoids, and essential oils rich in carvacrol and thymol. These phytochemicals exhibit significant antimicrobial, antioxidant, anti-inflammatory, and cytotoxic activities against various pathogens and disease models. Tissue culture-derived plants maintain comparable essential oil profiles to field-grown specimens, demonstrating the viability of in vitro propagation for commercial production. This review synthesizes current knowledge on micropropagation strategies and phytochemical characterization, highlighting the importance of C. amboinicus as a valuable medicinal plant resource and the role of biotechnological approaches in its conservation and sustainable utilization.
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Pages:33-39
How to cite this article:
Manju M George "<i>In vitro</i> propagation and phytochemical screening in <i>coleus amboinicus</i>". International Journal of Biology Research, Vol 6, Issue 4, 2021, Pages 33-39
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