The extensive use of pesticides in agriculture and for public health purposes, has led to drastic effects in many non-target species including man. In this study, the effect of cypermethrin and dimethoate mixture (uppercott) a popular agricultural pesticide on the kidney was investigated in male wistar albino rats. The rats were placed in five experimental groups with six (6) animals per group. Group 1 & 2 were taken as normal control and oil control respectively, given only standard feed and tap water, groups three (3) to five (5) were given 2.5%, 5% and 7.5% of uppercott LD₅₀ for 28 days. Results indicated that higher doses of uppercot (5% and 7.5%) significantly (p<0.05) increased serum urea compared to their controls while creatinine concentration was highest in the 7.5% group compared to control and other groups. In serum electrolytes, 2.5% group significantly (p<0.05) retained Na⁺ (sodium ion) compared to their controls (NCM and OCM), and also compared to 5% and 7.5% experimental groups respectively. Serum total proteins significantly (p<0.05) increased in 7.5% compared to controls and other test groups. The histology performed also helped confirm all the results as gotten from the biochemical assays. The study has led to the conclusion that uppercott pesticide may be a potential nephrotoxic agent.